Spike Protein (C-His-AVI, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

HEK 293

Tag

C-His-AVI

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

Immobilized Recombinant Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag) (Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-COV-2 EG.5.1 Spike RBD Protein (aa319-529, His&AVI Tag), Biotinylated (Cat: 40592-V49H15-B), the EC50 is 7-21 ng/mL.

Function

Arg319-lys529

Molecular Weight

The recombinant SARS-CoV-2 Spike RBD consists of 237 amino acids and predicts a molecular mass of 27.01 kDa. As a result of glycosylation, it migrates as an approximately 35.3 kDa band in SDS-PAGE under reducing conditions.