Spike Protein (C-His-AVI, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

HEK 293

Tag

C-His-AVI

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.

Bioactivity

Immobilized human ACE2 protein (Fc tag) (Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 (BA.2.12.1) Spike RBD Protein (His&AVI Tag), Biotinylated (Cat: 40592-V49H11-B), the EC50 is 4.5-13 ng/mL.

Function

Arg319-Phe541

Molecular Weight

The recombinant SARS-CoV-2 Spike RBD consists of 249 amino acids and predicts a molecular mass of 28.53 kDa. As a result of glycosylation, it migrates as an approximately 37.23 kDa band in SDS-PAGE under reducing conditions.