Spike Protein (C-His-AVI, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

HEK 293

Tag

C-His-AVI

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Bioactivity

Immobilized ACE2 Protein, Human, Recombinant (mFc Tag) (Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD (L452R, T478K) Protein (His & AVI Tag), Biotinylated (Cat: 40592-V49H1-B), the EC50 of SARS-CoV-2 Spike RBD (L452R, T478K) Protein (His & AVI Tag), Biotinylated (Cat: 40592-V49H1-B) is 8-48 ng/mL.

Function

Arg319-Phe541

Molecular Weight

The recombinant SARS-CoV-2 Spike RBD consists of 249 amino acids and predicts a molecular mass of 28.43 kDa. As a result of glycosylation, it migrates as an approximately 36.02 kDa band in SDS-PAGE under reducing conditions.