Spike Protein (C-His, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Immobilized Recombinant Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag) (Cat:10108-H05H) at 2μg/mL (100μL/well) can bind Recombinant SARS-CoV-2 CH.1.1 (Omicron) Spike RBD Protein (R319-K529, His Tag) (Cat: 40592-V08H150), the EC50 is 2.5-7.5 ng/mL.

Function

Arg319-Lys529

Molecular Weight

The recombinant SARS-CoV-2 Spike RBD consists of 222 amino acids and predicts a molecular mass of 25.30 kDa. As a result of glycosylation, it migrates as an approximately 33.67 kDa band in SDS-PAGE under reducing conditions.