Spike Protein (C-His, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95% as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.

Bioactivity

Immobilized human ACE2 protein (mFc tag) (Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 B.1.1.529 (Omicron) S1 Protein (His Tag) (Cat:40591-V08H41), the EC50 of is 45-140 ng/mL.

Function

Met1-Arg685

Molecular Weight

The recombinant SARS-CoV-2 Spike S1 consists of 678 amino acids and predicts a molecular mass of 76.48 kDa. As a result of glycosylation, it migrates as an approximately 99.56 kDa band in SDS-PAGE under reducing conditions.