Spike Protein (C-His, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

Immobilized human ACE2 protein (mFc tag) (Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike S1 (P9L, C136F, 144-145 deletion, R190S, D215G, Y449H, E484K, N501Y, D614G, H655Y, N679K) Protein (His Tag) (Cat:40591-V08H37), the EC50 is 12-80 ng/mL.

Function

Met1-Arg685

Molecular Weight

The recombinant SARS-CoV-2 Spike S1 consists of 679 amino acids and predicts a molecular mass of 76.06 kDa. As a result of glycosylation, it migrates as an approximately 114.84 kDa band in SDS-PAGE under reducing conditions.