HA Protein (C-His, H3N2)

Product Specifications

Abbreviation

Host

Baculovirus-insect Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System. 2. Measured by its ability to bind with Neu5Aca2-6GalNAca-PAA-biotin (01-059) using the Octet RED System. 3. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 1-5 ng/mL for 1% GRBC.

Function

Met1-Trp530

Molecular Weight

The recombinant hemagglutinin of Influenza A virus (A/California/07/2004 (H3N2) ) comprises 525 amino acids and has a predicted molecular mass of 59.2 kDa. The apparent molecular mass of the protein is approximately 70.6 kDa in SDS-PAGE under reducing conditions.