Spike Protein (C-His, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

HEK 293

Tag

C-His

Endotoxin

< 0.5 EU per mg protein.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

Immobilized Recombinant Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag) (Cat: 10108-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-COV-2 KP.2 (Omicron) Spike S1+S2 trimer Protein (ECD, His Tag) (Cat: 40589-V08H61-UE), the EC50 is 6-18 ng/mL (Routinely tested) .

Function

Met1-Gln1208

Molecular Weight

The recombinant SARS-CoV-2 (KP.2) Spike S1+S2 consists of 1227 amino acids and predicts a molecular mass of 136.43 kDa. It migrates as approximately 150-160 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 525.0 kDa verified by SEC-MALS (Routinely tested) .