Spike Protein (C-His, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.

Bioactivity

Immobilized human ACE2 protein (Fc tag) (Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 (BA.4.6/BF.7) Spike RBD Protein (His Tag) (Cat: 40592-V08H140), the EC50 is 19-65 ng/mL.

Function

Arg319-Phe541

Molecular Weight

The recombinant SARS-CoV-2 Spike RBD consists of 234 amino acids and predicts a molecular mass of 26.62 kDa. As a result of glycosylation, it migrates as an approximately 33.27 kDa band in SDS-PAGE under reducing conditions.