Spike Protein (C-His, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.

Bioactivity

1. Immobilized ACE2 Protein, Human, Recombinant (mFc Tag) (Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 B.1.1.529 sublineage BA.2 (Omicron) Spike RBD Protein (His Tag) (Cat:40592-V08H123), the EC50 is 5-30 ng/mL. 2. Load ACE2, mFc tag (Cat: 10108-H05H) on proA biosensor, can bind SARS-CoV-2 B.1.1.529 sublineage BA.2 (Omicron) Spike RBD Protein (Cat: 40592-V08H123) with an affinity constant of 2.3 nM as determined in BLI assay (ForteBio Octet Red384) (Routinely tested) .

Function

Arg319-Phe541

Molecular Weight

The recombinant SARS-CoV-2 Spike RBD consists of 234 amino acids and predicts a molecular mass of 26.71 kDa. As a result of glycosylation, it migrates as an approximately 36.55 kDa band in SDS-PAGE under reducing conditions.