Spike Protein (C-His, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE

Bioactivity

Immobilized human ACE2 protein (mFc tag) (Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind S4-2019-nCov-RBD (R346K, E484K, N501Y) (His tag) (Cat:40592-V08H118), the EC50 of S4-2019-nCov-RBD (R346K, E484K, N501Y) (His tag) (Cat:40592-V08H118) is 1-10 ng/mL.

Function

Arg319-Phe541

Molecular Weight

The recombinant SARS-CoV-2 Spike RBD consists of 234 amino acids and predicts a molecular mass of 26.56 kDa. As a result of glycosylation, it migrates as an approximately 34.86 kDa band in SDS-PAGE under reducing conditions.