VEGFR2/Flk-1/KDR Protein (C-hFc, Human)

Product Specifications

Abbreviation

VEGFR2/Flk-1/KDR

Synonyms

CD309, FLK-1, FLK1, Fetal liver kinase 1, Flk-1, KDR, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, VEGFR, VEGFR-2, VEGFR2, Vascular endothelial growth factor receptor 2

Host

HEK 293

Tag

C-hFc

Applications

Cell culture, ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

1. Immobilized recombinant human VEGFA at 1. 25 μg/ml (100 μl/well) can bind VEGFR2 with a linear range of 1.25-40.0 ng/ml. 2. Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC) . The ED50 for this effect is typically 20-120 ng/mL in the presence of 10 ng/mL recombinant human VEGF165.