TFPI Protein (C-His, Mouse)
Product Specifications
Abbreviation
TFPI
Synonyms
A630013F22Rik, AW552122, EPI, LACI
UniProt
O54819
Host
HEK 293
Tag
C-His
Applications
Enzyme
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 97 % as determined by reducing SDS-PAGE.
Bioactivity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp) -NH2 (Anaspec, Catalog # 27114) . The IC50 value is < 0.5 nM, as measured in 100μL reaction mixture containing 1. 25 ng trypsin (Sigma, Catalog # T4799), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij 35, pH 7.5.
Function
Met 1-Lys 289
Molecular Weight
31.0 kDa
Species
Mouse
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