SerpinA1/A1AT Protein (C-His, Human)

A1A, A1AT, AAT, Alpha-1 Protease Inhibitor, Alpha-1-Antiproteinase, Alpha-1-Antitrypsin, MGC23330, MGC9222, PI, PI1, PRO2275, SERPINA1, Serpin A1, alpha1AT

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp) -NH2 (Anaspec, Catalog#27114) . The IC50 value is < 3.0 nM, as measured in 100μL reaction mixture containing 1. 25 ng trypsin (Sigma, Catalog#T1426), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, pH 7.5.