TFPI Protein (C-His, Human)
Product Specifications
Abbreviation
TFPI
Synonyms
EPI, LACI, TFI, TFPI1
UniProt
P10646
Host
HEK 293
Tag
C-His
Applications
Enzyme
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 85 % as determined by reducing SDS-PAGE.
Bioactivity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp) -NH2 (Anaspec, Catalog#27114) . The IC50 value is < 0.4 nM, as measured in 100μL reaction mixture containing 1. 25 ng trypsin (Sigma, Catalog#T4799), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij-35, pH 7.5.
Function
Met 1-Lys 282
Molecular Weight
30.6 kDa
Species
Human
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