TFPI Protein (C-His, Human)

Product Specifications

Abbreviation

TFPI

Synonyms

EPI, LACI, TFI, TFPI1

UniProt

P10646

Host

HEK 293

Tag

C-His

Applications

Enzyme

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp) -NH2 (Anaspec, Catalog#27114) . The IC50 value is < 0.4 nM, as measured in 100μL reaction mixture containing 1. 25 ng trypsin (Sigma, Catalog#T4799), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij-35, pH 7.5.