TNFR1/TNFRSF1A Protein (C-His, Human)
Product Specifications
Abbreviation
TNFR1/TNFRSF1A
Synonyms
CD120a, FPF, MS5, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR1-d2, TNFR55, TNFR60, p55, p55-R, p60
UniProt
P19438
Host
HEK 293
Tag
C-His
Applications
Cell culture, ELISA
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 92 % as determined by reducing SDS-PAGE.
Bioactivity
1. Immobilized human TNFRI-His at 10 μg/mL (100 μl/well) can bind biotinylated human TNFα. The EC50 of biotinylated human TNFα is 5-12 μg/mL. 2. Measured by its ability to inhibit TNFα-mediated cytotoxicity in L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.2-1μg/mL in the presence of 0.25 ng/mL recombinant human TNFα.
Function
Met 1-Thr 211
Molecular Weight
22.7 kDa
Species
Human
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