Rat TNFR1/TNFRSF1A Protein (C-hFc, Rat)

Product Specifications

Abbreviation

Rat TNFR1/TNFRSF1A

Synonyms

TNFRSF1A, Tnfr-1, Tnfr1

UniProt

P22934

Host

HEK 293

Tag

C-hFc

Applications

Cell culture, ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

1. Measured by its ability to inhibit TNFα-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 ng/mL in the presence of 0.05 ng/mL of ratTNFα. 2. Immobilized mouse TNFa (80-235) at 10 μg/ml (100 μl/well) can bind rat TNFRSF1A-Fc, The EC50 of rat TNFRSF1A-Fc is 0.08-0.20 μg/ml.