TFPI2 Protein (C-His, Human)
Product Specifications
Abbreviation
TFPI2
Synonyms
PP5, Placental Protein 5, TFPI-2, TFPI2, Tissue Factor Pathway Inhibitor 2
UniProt
P48307
Host
HEK 293
Tag
C-His
Applications
Enzyme
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp) -NH2 (Anaspec, Catalog#27114) . The IC50 value is < 2 nM, as measured in 100μL reaction mixture containing 1. 25 ng trypsin (Sigma, Catalog#T4799), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15M NaCl, 0.05% Brij-35, pH 7.5.
Function
Met 1-Lys 213
Molecular Weight
23.2 kDa
Species
Human
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