SerpinF2/SERPINF2 Protein (C-His, Mouse)

Product Specifications

Abbreviation

SerpinF2/SERPINF2

Synonyms

Alpha-2-AP, Alpha-2-PI, Alpha-2-antiplasmin, Alpha-2-plasmin inhibitor, Serpin F2, Serpinf2

UniProt

Q61247

Host

HEK 293

Tag

C-His

Applications

Enzyme

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp) -NH2 (Anaspec, Catalog#27114) . The IC50 value is < 0.5 nM as measured in 100μL reaction mixture containing 1. 25 ng trypsin (Sigma, Catalog#T1426), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, pH 7.5.