Biotinylated FMC63 scFv, Fc, Avitag™ (MALS verified)

FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles.

Immobilized Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a at 1 μg/mL, add increasing concentrations of Rabbit Anti-Mouse FMC63 Polyclonal Antibody (Cat. No. FM3-S93) and then add Biotinylated FMC63 scFv, Fc,Avitag (Cat. No. CD9-BV4324b) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 78 ng/mL (QC tested).