Melatonin RIA Kit [CE]

Melatonin RIA kit measures melatonin by a double-antibody radioimmunoassay based on the Kennaway G280 anti-melatonin antibody. Reversed-phase column extracted samples and Controls and reconstituted Calibrators are incubated with the anti-melatonin antibody and 125I-melatonin. 125I-melatonin competes with melatonin present in samples, Calibrators and Controls. After 20 hours of incubation, solid-phase second antibody is added to the mixture in order to precipitate the antibody-bound fraction. Aft