Melatonin RIA Kit [CE]
Melatonin RIA kit measures melatonin by a double-antibody radioimmunoassay based on the Kennaway G280 anti-melatonin antibody. Reversed-phase column extracted samples and Controls and reconstituted Calibrators are incubated with the anti-melatonin antibody and 125I-melatonin. 125I-melatonin competes with melatonin present in samples, Calibrators and Controls. After 20 hours of incubation, solid-phase second antibody is added to the mixture in order to precipitate the antibody-bound fraction. Aft
Product Specifications
Certification
CE-IVD
Label
I125
Type
RIA
Sample Type
Serum - Plasma - Urine - Other biological fluids
Sample Volume
400 µl of extracted sample
Detection Range
0,5-50 pg/mL
Sensitivity
0,9 pg/ml
Format
RIA
Shelf Life
72 Weeks
Incubation Time
Overnight at 2-8°C + 15min at 2-8°C
Disease
Biogenic Amines and Neurosciences
Marker
Melatonin
Controls
2 levels
Pretreatment
1 ml of sample for extraction on columns
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