Vasoactive Intestinal Peptide (VIP) RIA [RUO]
VIPis analysed by the competitive radioimmunoassay using antibodies to a VIP-albumin conjugate. VIPin standards and samples compete with 125I-labelled VIP in binding to the antibodies. 125I-VIP binds in a reverse proportion to the concentration of VIP in standards and samples. In order to increase the sensitivity of the assay a sequential incubation is performed. Antibody-bound 125I-VIP is separated from the unbound fraction using the double antibody polyethylene glycol precipitation technique.
Product Specifications
Certification
RUO
Label
I125
Type
RIA
Sample Type
Plasma
Sample Volume
200 µl
Detection Range
3,8 - 120 pmol/L
Sensitivity
2,4 pmol/L
Format
RIA
Shelf Life
12 Weeks
Incubation Time
2x Overnight + 30min + 15min at 2-8C°
Disease
Adrenal Function & Hypertension
Marker
VIP
Controls
2 levels
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