TSH REC Ab ELISA [CE]

In the ELISA kit,TSH-R autoantibodiesin patient sera, calibrators and controls are allowed to interact withTSH receptors coated onto ELISA plate wells. After a 2 hour incubation, the samples are discarded leaving TRAb bound to the immobilised receptor. A human monoclonal autoantibody to the TSHR labelled with biotin (M22-biotin) is added in a second incubation step, where it interacts with immobilised TSH receptors which have not been blocked by bound TRAb. The amount of M22-biotin bound to the