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TNF-alpha ELISA [CE]

TheDiaSource TNF-α -ELISAis a solid phase Enzyme Amplified Sensitivity Immunoassay performed on microtiterplate. The assay uses monoclonal antibodies (MAbs) directed against distinct epitopes of TNF-α. Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1 – human TNF-α – MAb 2 – HRP, the mic

Product Specifications

Certification

CE-IVD

Label

HRP

Type

ELISA

Sample Type

Serum

Sample Volume

200 µL

Detection Range

7-518 pg/mL

Sensitivity

0,7 pg/mL

Format

ELISA

Shelf Life

60 Weeks

Incubation Time

4,5 h at RT with shaking 700 rpm

Disease

Inflammation Markers

Marker

TNF-a

Calibration

1 pg = 40 mIU of NIBSC IS 87/650

Controls

2 levels

Curated Selection

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