TNF-alpha ELISA [CE]
TheDiaSource TNF-α -ELISAis a solid phase Enzyme Amplified Sensitivity Immunoassay performed on microtiterplate. The assay uses monoclonal antibodies (MAbs) directed against distinct epitopes of TNF-α. Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1 – human TNF-α – MAb 2 – HRP, the mic
Product Specifications
Certification
CE-IVD
Label
HRP
Type
ELISA
Sample Type
Serum
Sample Volume
200 µL
Detection Range
7-518 pg/mL
Sensitivity
0,7 pg/mL
Format
ELISA
Shelf Life
60 Weeks
Incubation Time
4,5 h at RT with shaking 700 rpm
Disease
Inflammation Markers
Marker
TNF-a
Calibration
1 pg = 40 mIU of NIBSC IS 87/650
Controls
2 levels
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