17-OH Progesterone ELISA [CE]

The DiaSource17OH-Progesterone ELISAkit is a solid phase Enzyme Linked Immunosorbent Assay performed on microtiterplates. During a 1 hour incubation step, at room temperature, with shaking, 17-OHP present in calibrators, controls and samples competes with 17-OHP-HRP conjugate for binding sites of a specific antibody immobilized on the wells of the microtiterplate. After this incubation, microtiterplate is washed to stop the competition reaction. The Chromogenic solution (TMB) is added and incuba