AFP ELISA [CE]

The DiaSource AFP ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site on an AFP molecule. An aliquot of patient sample containing endogenous AFP is incubated in the coated well with enzyme conjugate, which is an anti- AFP antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bou