Anti-GFP (N86/38) Recombinant Chicken Chimeric mAb (BSA and Azide free)

Our chicken Anti-GFP recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N86/38. It detects GFP and is purified by affinity chromatography. It works in WB, IHC, ICC, IP, ELISA.

Product Specifications

Background

Green fluorescent protein (GFP) from the jellyfish Aequorea Victoria is widely used as a marker of protein expression or targeting in cells or model organisms.

Short Description

Our chicken Anti-GFP carrier-free recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N86/38. It detects n/a GFP and is purified by affinity chromatography. It works in WB, IHC, ICC, ELISA.

Synonyms

Green fluorescent protein

Gene Name

GFP

UniProt

P42212

Host

Chicken

Immunogen

Fusion protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein (accession number P42212) produced recombinantly in E. Coli

Target

GFP

Clonality

Recombinant

Clone

N86/38

Conjugation

Unconjugated

Type

Primary Antibodies

Applications

WB, IHC, ICC, ELISA

Field of Research

Anti-Tag Antibodies

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

pH

7.4

Molecular Weight

27 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N86/38 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N86/38 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and buffer exchanged into 1X PBS.

IHC Dilution

1:500-1:1000

WB Dilution

1:1000-1:3000

Specificity

Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively) Does not cross-react with Red fluorescent protein (dsRed)

ICC Dilution

1:500-1:1000

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Brand

Aves Labs

Physical Properties

Liquid

Isotype

IgY

Immunogen Species

Jellyfish

ICC Quality Control

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