Anti-GFP (N86/38) Recombinant Chicken Chimeric mAb

Our chicken Anti-GFP recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N86/38. It detects GFP and is purified by affinity chromatography. It works in WB, IHC, ICC, IP, ELISA.

Product Specifications

Background

Green fluorescent protein (GFP) from the jellyfish Aequorea Victoria is widely used as a marker of protein expression or targeting in cells or model organisms.

Short Description

Our chicken Anti-GFP recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N86/38. It detects n/a GFP and is purified by affinity chromatography. It works in WB, IHC, ICC, IP, ELISA.

Synonyms

Green fluorescent protein

Gene Name

GFP

UniProt

P42212

Host

Chicken

Immunogen

Fusion protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein (accession number P42212) produced recombinantly in E. Coli

Target

GFP

Clonality

Recombinant

Clone

N86/38

Conjugation

Unconjugated

Type

Primary Antibodies

Applications

WB, IHC, ICC, IP, ELISA

Field of Research

Anti-Tag Antibodies

Concentration

1 mg/mL

Format

Purified by affinity chromatography

pH

7.4

Molecular Weight

27 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N86/38 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N86/38 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.