Anti-GluN1/NR1 Glutamate Receptor (N308/48) Recombinant Chicken Chimeric mAb

Glutamate receptor 1, NMDA 1, or GluN1/NR1 also known as subunit 1 of the N-methyl-D-aspartate (NMDA) receptor. It is a member of the glutamate receptor channel superfamily and is a heteromeric glutamate-gated calcium ion channel. GluN1 is essential for synaptic function in the brain. GluN1 is expressed throughout the brain in cerebellum, thalamus, olfactory bulb and cortex in the membrane at postsynaptic density. GluN1 plays a key role in synaptogenesis, synaptic plasticity, memory acquisition and learning. Mutations in the GluN1 gene are associated with intellectual disability and cortical visual impairment and movement disorders.

Our chicken Anti-GluN1/NR1 glutamate receptor recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N308/48. It detects human, mouse, and rat GluN1/NR1 glutamate receptor and is purified by affinity chromatography. It works in WB, IHC, ICC, ELISA.

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N308/48 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N308/48 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Each new lot of antibody is quality control tested by Western blot on rat brain membrane fraction and confirmed to stain the expected molecular weight band.