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Anti-GluA2/GluR2 Glutamate Receptor (L21/32) Recombinant Chicken Chimeric mAb FL650 Conjugate

Our chicken Anti-GluA2/GluR2 glutamate receptor FL650 conjugate recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone L21/32. It detects human, mouse, and rat GluA2/GluR2 is purified by affinity chromatography. It works in IHC, ICC.

Product Specifications

Background

Glutelin type-A 2 (GluA2), also called GRIA2, GLUR2, GLURB, GluA2, GluR-K2, HBGR2 or glutamate ionotropic receptor AMPA type subunit 2, is a member of the Glutamate receptor family of the mammalian brain. This neurotransmiter receptor subunit, which is activated during normal central nervous system fuction, is encoded by the GRIA2 (or GLUR2) gene. GluA2 constitutes a key subunit that regulates AMPA receptors. AMPA receptors lacking of Glua2 have been shown to be present in diseased brains, whose basic fuctions have been altered.

Short Description

Our chicken Anti-GluA2/GluR2 glutamate receptor FL650 conjugate recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone L21/32. It detects human, mouse, and rat GluA2/GluR2 glutamate receptor and is purified by affinity chromatography. It works in IHC, ICC.

Synonyms

Glutamate receptor 2 (GluR-2) (AMPA-selective glutamate receptor 2) (GluR-B) (GluR-K2) (Glutamate receptor ionotropic, AMPA 2) (GluA2)

Gene Name

Gria2 Glur2

UniProt

P19491

Host

Chicken

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 834-883 (EFCYKSRAEAKRMKVAKNPQNINPSSSQNSQNFATYKEGYNVYGIESVKI, cytoplasmic C-terminus) of rat GluA2/GluR2 produced recombinantly in E. Coli

Target

GluA2/GluR2 glutamate receptor

Clonality

Recombinant

Clone

L21/32

Conjugation

FL650 Ex: 655 nm, Em: 676 nm

Type

Primary Antibodies

Applications

IHC, ICC

Field of Research

Cell Markers / Neuronal Markers / Synapse Markers / Post-synaptic Markers / Neurotransmitter Receptors / Glutamate Receptors

Concentration

0.5 mg/mL

Format

Purified by affinity chromatography

pH

7.4

Molecular Weight

90 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L21/32 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L21/32 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.

IHC Dilution

1:250-1:500

Specificity

Does not cross-react with GluA1/GluR1, GluA3/GluR3 or GluA4/GluR4 (based on KO validation results)

ICC Dilution

1:500

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat brain membrane fraction and confirmed to stain the expected molecular weight band.

Brand

Aves Labs

Physical Properties

Liquid

Isotype

IgY

Immunogen Species

Rat

WB Brain Quality Control

1

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