Anti-Aldh1L1 (N103/39) Recombinant Chicken Chimeric mAb

Our chicken Anti-Aldh1L1 recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N103/39. It detects human, mouse, non-human primate, and rat Aldh1L1, and is purified by affinity chromatography. It is great for use in ELISA, ICC, IHC, WB.

Product Specifications

Background

Aldehyde dehydrogenase family 1 member L1 (Aldh1L1) is a member of the aldehyde dehydrogenase family. This protein is an enzyme that catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. It is an astrocyte marker and also is expressed in the liver. It is proposed to play a role in regulation of cellular THF levels and inhibiting cell proliferation. Alterations in Aldh1L1 have been implicated in Congenital Hydrocephalus

Short Description

Synonyms

Cytosolic 10-formyltetrahydrofolate dehydrogenase (10-FTHFDH) (FDH) (EC 1.5.1.6) (Aldehyde dehydrogenase family 1 member L1) (FBP-CI)

Gene Name

Aldh1l1 Fthfd

UniProt

P28037

Host

Chicken

Reactivity

Human, Mouse, Non-Human Primate, and Rat

Immunogen

Protein amino acids 1-902 (full-length) of rat Aldh1L1 (accession number P28037) produced recombinantly in E. Coli

Target

Aldh1L1

Clonality

Recombinant

Clone

N103/39

Conjugation

Unconjugated

Type

Primary Antibodies

Applications

ELISA, ICC, IHC, WB

Field of Research

Cell Markers / Glial and Schwann Cell Markers / Astrocyte Markers

Concentration

1 mg/mL

Format

Purified by affinity chromatography

pH

7.4

Molecular Weight

100 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions clone N103/39 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N103/39 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

IHC Dilution

1:250-1:1000

WB Dilution

1:1000-1:2000

Specificity

No cross-reactivity against Aldh1L2

ICC Dilution

1:1000

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.