ELISA Kit for Alpha-1-Antitrypsin (a1AT)

SERPINA1; SPAAT; A1-AT; PI; Serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase/AntiTrypsin) Member 1; Alpha-1 protease inhibitor; Short peptide from AAT; Serpin A1

This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to a1AT has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled a1AT and unlabeled a1AT (Standards or samples) with the pre-coated antibody specific to a1AT. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of a1AT in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of a1AT in the sample.

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Alpha-1-Antitrypsin (a1AT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Alpha-1-Antitrypsin (a1AT) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

This assay has high sensitivity and excellent specificity for detection of Alpha-1-Antitrypsin (a1AT) . No significant cross-reactivity or interference between Alpha-1-Antitrypsin (a1AT) and analogues was observed.