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Mouse Multi-Neurotrophin Screening Rapid ELISA Assay

Product Specifications

Background

The Neurotrophin family of growth factors in all mammals including human has four members including Nerve Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF), Neurotrophin 3 (NT3) and Neurotrophin 4/5 (NT4/5) . These are all essential to brain development, maturation and adult function, particularly for cell differentiation, survival, death and synaptic regulation.

Short Description

The Biosensis Multi-Neurotrophin Rapid Screening ELISA kit has been designed to allow rapid screening and quantification of mouse NGF, BDNF, NT3 and NT4/5 in cell culture supernatants, lysates and brain extracts. This two-plate kit consists of four sets of 6 strips for each Neurotrophin, allowing for the assay of 16 samples per Neurotrophin tested and a full range of standards, all run in duplicate. It provides the identical sensitivities and ranges that are achieved in the complete, individual ELISA kits, allowing easy progression into the larger individual assay sizes when needed. The Multi-Neurotrophin RapidTM Screening ELISA kit presents a cost-effective way to quickly screen multiple samples. It comes complete with all detection reagents and is ready to use.

Synonyms

NGF; BDNF; NT3; NT4/5

UniProt

P21237; P01139; P20181; Q80VU4

Reactivity

Mouse

Immunogen

As for individual ELISA kits

Target

Multi-Neurotrophin Screening

Applications

ELISA

Sample Type

Cell Lysates, Culture Supernatant, Tissue Homogenates

Precautions

For research use only.

Shipping Conditions

Shipped on ice packs

Storage Conditions

Store at 2-8°C

Specificity

This Multi-Neurotrophin Screening kit uses the same antibodies as in the individual Rapid ELISA kits. Each kit has been tested for cross-reactivity with other closely related neurotrophins and no cross-reactivity has been observed at 25 ng/mL of each neurotrophin tested. The antibodies used in this kit detect the human and rat form of each neurotrophin, but with varying efficiency depending on the target protein.

Method

Colorimetric

Immunogen Species

Mouse

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