HQ-O RTD Amyloid Plaque Stain Reagent

Product Specifications

Background

A novel zinc chelator, HQ-O, was developed for localizing zinc within amyloid plaques. The histology involves incubating tissue sections in a dilute aqueous solution of HQ-O.

Short Description

HQ-O Ready-To-Dilute (RTD) Stain Reagent is designed to label amyloid plaques in paraffin-embedded or freshly cut frozen tissue sections.

Synonyms

HQ-O tracer

Reactivity

Human, Mouse, Other Mammals (Predicted), Rat

Target

Amyloid plaque

Conjugation

To visualize the HQ-O tracer, it is recommended to use a filter cube designed for visualizing Fluorescein/FITC or a blue-light laser. Although it can be seen with both narrow and wide-band pass filters, there is no need to use a narrow band filter since the compound does not "bleed through" when excited with other filters. A recommended excitation range of a wide band filter is 447 - 503 nm, with a peak at 475 nm.

Applications

IHC-Frozen, IHC-Paraffin-embedded

Concentration

10X

Format

Purified

Precautions

For research use only.

Shipping Conditions

Shipped on ice packs

Storage Conditions

Store 10X stock solution at 2-8°C protected from light, for up to 6 months. The diluted dye (1X) should be used within 24 hours.

Specificity

As a fluorescent zinc chelator, HQ-O is unique as it takes advantage of the known presence of concentrated zinc in amyloid plaques. Studies with HQ-O revealed that fluorescent plaque-like structures are only seen when synthetic Aβx-42 is aggregated in the presence of zinc. Under blue light excitation, plaque structures appear bright green fluorescent in the brain parenchyma, correlating closely with plaque structures observed following Aβ antibody staining.