LipoFluor-DMN-DTTM Lipid Droplets & ER Positive Vesicle Tracing Reagent

Product Specifications

Short Description

LipoFluor-DMN-DTTM is a new fluorescent imaging agent that can discern both LDs (lipid droplets) and ER (endoplasmic reticulum) compartments using a single excitation wavelength.

Synonyms

DMN-DT, 3,4-dimethoxy-1,8-naphthalimide, N- (2- (5,6-Dimethoxy-1,3-dioxo-1H-benzo[de]isoquinolin-2 (3H) -yl) ethyl) -4-methylbenzenesulfonamide

Reactivity

Human, Mouse, Other Mammals, Rat

Target

Lipid Droplets & Endoplasmic Reticulum

Conjugation

λex 403 nm. For regions of LD staining, the emission maximum was centered at 456 nm (blue light), while this was red-shifted for the ER (λem 486 nm), a more (blue-green) light emission. Dye is solvatochromic, meaning that is emission changed depending upon is micro environment.

Applications

IF, ICC, Live Cell IF

Sample Type

Live cell culture and fixed brain tissue, other samples not yet tested

Concentration

100X-2000X

Buffer

DMSO

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

The liquid stock solution vials can be stored up to 12 months from date of receipt at 2-8°C, properly sealed and protected from light. Working solutions should be used within 24 hours and not stored for later use.

Notes

Live cell culture and fixed brain tissue. Validated Cell lines: LipoFluor-DMN-DT TM to stain multiple cell lines, a range of malignant (22Rv1, LNCaP, PC3, and HCT-8) and non-malignant (PNT1a and PNT2) cell types. Other cell lines are expected to stain as well when alive.

Specificity

LipoFluor-DMN-DT TM is a new fluorescent imaging agent that can discern both LDs (lipid droplets) and ER (endoplasmic reticulum) compartments using a single excitation wavelength.