LipoFluor-ER2TM Endoplasmic Reticulum Blue Tracing Reagent

LipoFluor-ER2TM is a cell-permeant stain for the endoplasmic reticulum with unique spectral properties, which makes it particularly useful for antibody double- and triple-labelling experiments. LipoFluor-ER2TM is highly compatible with green, red and far-red fluorophore-labelled antibodies and has been successfully used to image the endoplasmic reticulum in human and other mammalian cell lines.

The liquid stock solution vials can be stored up to 12 months from date of receipt at 2-8°C, properly sealed and protected from light. Working solutions should be used within 24 hours and not stored for later use.

Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of fluorescent isomers. No amount of starting material was detected.

Fluorescent imaging of the endoplasmic reticulum compartment. LipoFluor-ER2TM localizes to the endoplasmic reticulum in a variety of live and fixed eukaryotic cells. It is highly cell permeable and produces fast results with ease and may be used in conjunction with antibody staining. The dye, however, cannot be fixed within the cells with post fixation methods.