LipoFluor-P2TM Polar Lipid & Endoplasmic Reticulum Tracing Reagent

Product Specifications

Short Description

LipoFluor-P2TM is a cell-permeant stain for intracellular lipid droplets and the endoplasmic reticulum. This tracing reagent can be used on live and fixed cells and is compatible with other dyes.

Synonyms

LipoFluor-P2TM

Reactivity

Human, Mouse, Other Mammals

Target

Endoplasmic Reticulum Lipids & Polar Lipid Molecules

Conjugation

Ex/Em: 405 nm / 600 nm

Applications

IF, ICC, Live Cell IF

Assay Protocol

Each vial can be diluted 100X to 2000X depending upon the final working concentration of the dye in the experiment. Typical working concentrations for LipofluorTM TR-603-P2 are 10μM-20μM

Sample Type

Cells, Tissues (live or fixed)

Concentration

100X-2000X

Buffer

DMSO

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

The liquid stock solution vials can be stored up to 12 months from date of receipt at 2-8°C, properly sealed and protected from light. Working solutions should be used within 24 hours and not stored for later use.

Notes

Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of fluorescent isomers. No amount of starting material was detected.

Specificity

Fluorescent imaging of lipid-rich cellular compartments. Cell penetration and localization of LipoFluor-P2TM has been confirmed in cardiomyocytes, H9c2 cell line and LNCaP prostate cells.