LipoFluor-ER1TM Endoplasmic Reticulum Tracing Reagent

LipoFluor-ER1TM is a cell-permeant stain for the endoplasmic reticulum. LipoFluor-ER1TM passively diffuses across the plasma membrane into the cell, stains at low concentrations and has minimal cytotoxic effects.

Each vial can be diluted 100X to 2000X depending upon the final working concentration of the dye in the experiment. Typical working concentrations for LipofluorTM LipofluorTM TR-601-ER1 are 10μM-20μM.

The liquid stock solution vials can be stored up to 12 months from date of receipt at 2-8°C, properly sealed and protected from light. Working solutions should be used within 24 hours and not stored for later use.

Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of fluorescent isomers. No amount of starting material was detected.

Fluorescent imaging of the endoplasmic reticulum compartment. Cell penetration and localisation of LipoFluor-ER1TM has been confirmed in a range of cell lines, including prostate cells (PNT2, PNT1a, LNCaP, 22RV1 and DU145), cardiomyocytes (H9c2) and neuronal cells (PC-12) .