LipoFluor-P1TM Polar Lipid Tracing Reagent

LipoFluor-P1TM is a cell-permeant stain with an affinity for polar lipids which can be used in a wide variety of live and fixed samples. LipoFluor-P1TM provides excellent and rapid lipid staining used for fluorescent microscopy applications and automated imaging.

Each vial can be diluted 100X to 2000X depending upon the final working concentration of the dye in the experiment. Typical working concentrations for Lipofluor-P1TM TR-600-P1 are 10μM-50μM.

The liquid stock solution vials can be stored up to 12 months from date of receipt at 2-8°C, properly sealed and protected from light. Working solutions should be used within 24 hours and not stored for later use.

Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of fluorescent isomers. No amount of starting material was detected.

Fluorescent imaging of lipid-rich compartments. LipoFluor-P1TM is an effective tracer of lipids (e.g., cholesterol, sphingolipid, and phospholipid) and intracellular localization. Cell penetration and localisation of LipoFluor-P1TM has been confirmed in a range of cell lines; including adipocytes (3T3-L1), prostate cells (PNT2, PNT1a, LNCaP, 22RV1 and DU145), cardiomyocytes (H9c2) and neuronal cells (PC-12), and tissues; including adipose tissue (sheep and Drosophila), muscle tissue (sheep cardiac and skeletal) and brain (murine) .