Fluoro-Jade C (FJC) Staining Kit

The Fluoro-Jade C Ready-to-Dilute (RTD) Staining Kit provides an easy to use assortment of Fluoro-Jade C, DAPI, sodium hydroxide and potassium permanganate in liquid form. The Fluoro-Jade C Staining Kit can be used on all kinds of preserved tissues, including fresh-frozen, paraformaldehyde or formalin fixed, and formalin fixed, paraffin-embedded tissues.

FJC visualization is accomplished using blue light or a 488 nm Laser. Excitation Peak: 495 nm Emission Peak: 521 nm Filter system for visualizing: Fluorescein/FITC

Dilute solutions as directed in the protocol instructions. Sometimes small precipitates may be present in the stock or diluted solutions. Complete mixing of the diluted solutions usually dissolves the precipitates. The precipitates, if not removed, do not usually cause any difficulties if the washing steps are followed as instructed. Optional: For entirely clean solutions Biosensis recommends filtering the diluted solution though ethanol and NaOH compatible syringe or vacuum filter devices prior to contact with tissue slides.

Degenerating neurons, and neuronal degeneration. There is no specific staining in normal healthy brain. Note: Some researchers under some conditions report blood vessel staining with Fluoro Jade. This may be because Fluoro Jade is an analogue of eosin (which stains blood cells) . In general, good perfusion and preparation of the tissue should help prevent blood vessel staining but it may not be possible to eliminate it entirely. In our experience it is generally possible to distinguish neuronal from blood vessels staining by eye.