NGFR/p75NTR-Fc Chimera, Mouse, Purified Recombinant Protein

Nerve growth factor (NGF) receptor, also known as p75NTR, is a low affinity NGF receptor. It binds with equal affinity all members of the neurotrophin family including beta NGF, BDNF, NT-3 and NT-4/5. It also binds the pro-neurotrophins. NGF receptors mediate signaling of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. These receptors are also thought to play a role in neurogenerative diseases such as Alzheimers disease. The p75NTR NGF receptor is a type I transmembrane glycoprotein (396 aa) consisting of a signal peptide (21 aa), an extracellular domain (225 aa) which contains four cysteine rich domains responsible for ligand binding, a transmembrane domain (19 aa) and a cytoplasmic domain (152 aa) . It is a member of the TNF-alpha receptor family (TNR16) . Recently, p75NTR has been shown to act as a receptor for many pathogens such as prions, rabies virus and amyloid beta. It also acts as a co-receptor for NOGO, mediating inhibitory signals of myelin associated protein.

Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; p75NTR; CD271; Fast NGF receptor.

A DNA sequence encoding the signal peptide and extracellular domain of human NGF receptor (amino acids 1-224) was fused to the Fc region of human IgG1 (amino acids 104-330) . The chimeric protein was expressed in modified CHO cells. The full sequence is available on request from biospeak@biosensis.com.

It is recommended that 0.25 mL of sterile phosphate-buffered saline (PBS) be added to the vial. Lyophilized products should be stored at 2-8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.