Stem cell factor, Human, Purified Recombinant Protein
Product Specifications
Background
Stem Cell Factor (SCF) is a hematopoietic growth factor that stimulates the proliferation of mast cells. SCF also mediates cell-cell adhesion and acts synergistically with other cytokines. The actions of SCF are exerted by binding to the SCF Receptor (PE-1243-50) which results in receptor dimerization. SCF is a type I membrane glycoprotein with 5 potential N-linked glycosylation sites. Soluble SCF is produced by proteolytic cleavage of the extracellular domain.
Short Description
Stem cell factor, purified human recombinant protein, suitable for cell culture.
Synonyms
Kit ligand; C-kit ligand; Stem cell factor; SCF; Mast cell growth factor; MGF; KITLG; MGF; SCF
UniProt
P21583
Target
Stem cell factor
Sequence
A DNA sequence encoding the signal peptide and the mature Stem Cell Factor (SCF) protein of human SCF was expressed in modified human 293 cells.
Applications
Cell Culture
Format
Purified SCF consists of 0-50% carbohydrate by weight.
Buffer
When reconstituted in 0.5 mL sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Precautions
For research use only.
Shipping Conditions
25°C (ambient)
Storage Conditions
Reconstitute with sterile-filtered phosphate-buffered saline to a concentration of 20 μg/mL. Lyophilized products should be stored at 2-8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Notes
>95% purity, as determined by SDS-PAGE and visualized by silver stain
Host or Source
HEK293
Physical Properties
Lyophilized
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