Hepatocyte growth factor (HGF), Human, Purified Recombinant Protein
Product Specifications
Background
Hepatocyte growth factor (HGF) is a member of the plasminogen related growth factor family. The HGF protein is a dimer formed from an alpha and beta subunit linked by a disulfide bond. Both subunits originate from the endoproteolytic cleavage of a common precursor which is biologically inactive. HGF is expressed in Kupffer cella and sinusoidal endothelial cells in the liver and pancreas.
Short Description
Hepatocyte growth factor (HGF), purified human recombinant protein, suitable for cell culture.
Synonyms
HGF; HPTA; Scatter factor; SF; Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain; Hepatopoeitin-A
UniProt
P14210
Target
Hepatocyte growth factor (HGF)
Sequence
A DNA sequence encoding the complete human Hepatocyte Growth Factor protein isoform 1 (comprising the signal peptide, alpha and beta chains) was expressed in modified human 293 cells.
Applications
Cell Culture
Format
Purified. The HGF formulation consists of 0-30% carbohydrate by weight.
Buffer
When reconstituted in 0.5 mL sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Precautions
For research use only.
Shipping Conditions
25°C (ambient)
Storage Conditions
It is recommended that 0.5 mL of sterile phosphate-buffered saline be added to the vial. Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 2-8°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Notes
>95% purity, as determined by SDS-PAGE and visualised by Coomassie Brilliant Blue
Host or Source
HEK293
Physical Properties
Lyophilized
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