Anti-Neurofilament light polypeptide (NF-L), DG-SensorTM Antibody

Our Anti-Neurofilament light polypeptide (NF-L) [degenerated] chicken polyclonal primary antibody detects bovine, human, mouse, pig, and rat Neurofilament light polypeptide (NF-L) [degenerated], and is igg. It is validated for use in IF, IHC-non-Paraffin-embedded, WB.

Product Specifications

Background

Caution:

Short Description

Our Anti-Neurofilament light polypeptide (NF-L) [degenerated] chicken polyclonal primary antibody detects bovine, human, mouse, pig, and rat Neurofilament light polypeptide (NF-L) [degenerated], and is IgG. It is validated for use in IF, IHC-non-Paraffin-embedded, WB.

Synonyms

NFL, Neurofilament light polypeptide, DG-Sensor, NF-L degenerative, NEFL, CMT1F, CMT2E, NF-L, NF68, PPP1R110, neurofilament light, CMTDIG, neurofilament light chain

UniProt

P07196

Reactivity

Bovine, Human, Mouse, Pig, and Rat

Immunogen

The antibody has been made against a proprietary recombinant construct containing amino acids of human NF-L expressed in and purified from E Coli.

Target

Neurofilament light polypeptide (NF-L) [degenerated]

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

IF, IHC-non-Paraffin-embedded, WB

Format

IgG

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute with 100 μL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.

Notes

Affinity-purified

IHC Dilution

1:1000-1:10000

WB Dilution

1:1000-1:5000

Specificity

Species cross-reactivity includes human, rat, mouse, cow, and pig. These are antibodies which recognize epitopes in a small segment of the neurofilament NF-L subunit which are normally not accessible to antibodies but which became available on degeneration in natural or native conditions. Under reduced or proteinase treated tissues or Western blot, these antibodies are all specific for NF-L from a variety of species.