Anti-Neurofilament light polypeptide (NF-L) Antibody (DA2)

Our Anti-Neurofilament light polypeptide (NF-L) mouse monoclonal primary antibody detects bovine, horse, human, mouse, pig, and rat Neurofilament light polypeptide (NF-L), and is igg. It is validated for use in FC, IF, ICC, IHC, WB.

Product Specifications

Background

Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins. (Ref: uniprot.org)

Short Description

Our Anti-Neurofilament light polypeptide (NF-L) mouse monoclonal primary antibody detects bovine, horse, human, mouse, pig, and rat Neurofilament light polypeptide (NF-L), and is IgG. It is validated for use in FC, IF, ICC, IHC, WB.

Synonyms

NF-L; NF68; NEFL; Neurofilament light polypeptide; Neurofilament protein; Neurofilament triplet L protein; NFL

UniProt

P07196

Reactivity

Bovine, Horse, Human, Mouse, Pig, and Rat

Immunogen

The antibody has been made against enzymatically dephosphorylated, full length, pig NF-L protein. The antibody binding epitope has been mapped to a short peptide in the C-terminal â€œtailâ€ region of the molecule within the sequence YYTSHVQEEQIEVEETIEA, amino acids 441-460 of the human sequence.

Target

Neurofilament light polypeptide (NF-L)

Clonality

Monoclonal

Clone

DA2

Conjugation

Unconjugated

Applications

FC, IF, ICC, IHC, WB

Format

IgG

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute with 50 μL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.