Anti-Vimentin Antibody
Our Anti-Vimentin mouse monoclonal primary antibody detects human and rat Vimentin, and is igg. It is validated for use in ICC, IHC-Frozen, WB.
Product Specifications
Background
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.Vimentins are Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. (Ref: uniprot.org)
Short Description
Our Anti-Vimentin mouse monoclonal primary antibody detects human and rat Vimentin, and is IgG. It is validated for use in ICC, IHC-Frozen, WB.
Synonyms
Vimentin, VIM
UniProt
P08670
Reactivity
Human and Rat
Immunogen
The antibody has been made against the full length recombinant human vimentin protein expressed in and purified from E Coli. The antibody binds to a region in the C-terminal “tail†region of vimentin included in the peptide SRISLPLPNFSSLNLRE.
Target
Vimentin
Clonality
Monoclonal
Clone
2A52
Conjugation
Unconjugated
Applications
ICC, IHC-Frozen, WB
Format
IgG
Buffer
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
Precautions
For research use only.
Storage Conditions
Spin vial briefly before opening. Reconstitute with 100 μL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Notes
Protein G purified
IHC Dilution
1:100-1:500
WB Dilution
1:5000-1:10000
Specificity
Human. Species cross-reactivity includes rat.
ICC Dilution
1:100-1:500
Host or Source
Mouse
Physical Properties
Lyophilized
Isotype
IgG1
Immunogen Species
Human
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