Anti-Green Fluorescent Protein Tag (GFP) Antibody

Our Anti-Green fluorescent protein (GFP) chicken polyclonal primary antibody detects species independent Green fluorescent protein (GFP), and is affinity-purified. It is validated for use in ICC, WB.

Product Specifications

Short Description

Our Anti-Green fluorescent protein (GFP) chicken polyclonal primary antibody detects Green fluorescent protein (GFP), and is affinity-purified. It is validated for use in ICC, WB.

Synonyms

Green fluorescent protein, GFP

UniProt

Q6YGZ0

Reactivity

Species Independent

Immunogen

Recombinant AcGFP protein expressed in and purified from E. Coli.

Target

Green fluorescent protein (GFP)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ICC, WB

Format

Affinity-purified

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Molecular Weight

27 kDa

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute with 100 μL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) . Avoid repetitive freeze/thaw cycles.

WB Dilution

1:1000-1:5000

Specificity

Antibody recognizes GFP protein in GFP-transfected HEK293 cells, but not in non-transfected control cells. Specific for GFP, does not cross-react with mCherry.