Anti-Brain-derived neurotrophic factor (BDNF) Antibody

Our Anti-Brain-derived neurotrophic factor (BDNF) rabbit polyclonal primary antibody detects human, mouse, other mammals (predicted), and rat Brain-derived neurotrophic factor (BDNF), and is igg. It is validated for use in ELISA, IHC-Frozen, WB.

Product Specifications

Short Description

Our Anti-Brain-derived neurotrophic factor (BDNF) rabbit polyclonal primary antibody detects human, mouse, other mammals (predicted), and rat Brain-derived neurotrophic factor (BDNF), and is IgG. It is validated for use in ELISA, IHC-Frozen, WB.

Synonyms

Brain-derived neurotrophic factor; Abrineurin; proBDNF

UniProt

P23560

Reactivity

Human, Mouse, Other Mammals (Predicted), and Rat

Immunogen

A synthetic peptide (HSDPARRGEL) as a part of human BDNF protein (aa: 129-138) conjugated to KLH has been used as the immunogen. The BDNF protein sequence is highly conserved amongst mammalian species.

Target

Brain-derived neurotrophic factor (BDNF)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, IHC-Frozen, WB

Assay Protocol

Technical Note #4 - Acid-Extraction of BDNF From Brain Tissue Instructions for Acid-Extraction of Brain Tissue BDNF Antibodies for Western Blotting

Format

IgG

Buffer

Lyophilized from PBS, pH 7.4, without preservatives.

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute in 500 μL sterile-filtered ultrapure water, pH 7.2-7.6. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Notes

Protein G purified IgG

IHC Dilution

1-10 μg/mL

WB Dilution

1-10 μg/mL

Specificity

Less than 0.1% cross reactivity with mouse NGF, recombinant human NT3 and NT4/5 has been recorded by dot blot analysis. This antiserum is known to recognize rat, human and human BDNF, and is expected to react with BDNF from other species due to amino acid sequence homology.